Human remains have been discovered in the wreckage of the Titan submersible that imploded, claiming the live of five people onboard.

US medical professionals say they will now conduct a formal analysis of “presumed human remains that have been carefully recovered” within the wreckage at the site of the incident.

Debris and wreckage from the submersible was recovered from the seabed overnight.

The debris has been returned to land after a fatal implosion during its voyage to the wreck of the Titanic captured the world’s attention last week.

The return of the debris to port in St John’s, Newfoundland and Labrador, is a key piece of the investigation into why the submersible imploded, killing all five people on board. Twisted chunks of the 6.7m submersible were unloaded at a Canadian Coast Guard pier on Wednesday.

Horizon Arctic, a Canadian ship, carried a remotely operated vehicle, or ROV, to search the ocean floor near the Titanic wreck for pieces of the submersible. Pelagic Research Services, a company with offices in Massachusetts and New York that owns the ROV, said on Wednesday that it has completed offshore operations.

Pelagic Research Services’ team is “still on mission” and cannot comment on the ongoing Titan investigation, which involves several government agencies in the US and Canada, said Jeff Mahoney, a spokesman for the company.

Debris from the Titan submersible is unloaded at the Canadian Coast Guard pier in St. John's, Newfoundland. Photo / Paul Daly, The Canadian Press via AP

“They have been working around the clock now for 10 days, through the physical and mental challenges of this operation, and are anxious to finish the mission and return to their loved ones.”

Debris from the Titan was located about 3810m underwater and roughly 488m from the Titanic on the ocean floor, the Coast Guard said last week. The Coast Guard is leading the investigation into why the submersible imploded during its June 18 descent. Officials announced on June 22 that the submersible had imploded and all five people on board were dead.

The Coast Guard has convened a Marine Board of Investigation into the implosion. That is the highest level of investigation conducted by the Coast Guard.

One of the experts the Coast Guard consulted with during the search said analysing the physical material of recovered debris could reveal important clues about what happened to the Titan. And there could be electronic data, said Carl Hartsfield of the Woods Hole Oceanographic Institution.

“Certainly all the instruments on any deep sea vehicle, they record data. They pass up data. So the question is, is there any data available? And I really don’t know the answer to that question,” he said Monday.

Workers handle debris from the Titan submersible at the Canadian Coast Guard pier in St. John's. Photo / Paul Daly, The Canadian Press via AP

Representatives for Horizon Arctic did not respond to requests for comment.

Coast Guard representatives declined to comment on the investigation or the return of debris to shore on Wednesday. Representatives for the National Transportation Safety Board and Transportation Safety Board of Canada, which are both involved in the investigation, also declined to comment.

The National Transportation Safety Board has said the Coast Guard has declared the loss of the Titan submersible to be a “major marine casualty” and the Coast Guard will lead the investigation.

 “We are not able to provide any additional information at this time as the investigation is ongoing,” said Liam MacDonald, a spokesman for the Transportation Safety Board of Canada.

A spokesperson for the International Maritime Organization, the UN’s maritime agency, has said any investigative reports from the disaster would be submitted for review. Member states of the IMO can also propose changes such as stronger regulations of submersibles.

Currently, the IMO has voluntary safety guidelines for tourist submersibles which include requirements they be inspected, have emergency response plans, and have a certified pilot on board among other requirements. Any safety proposals would not likely be considered by the IMO until its next Maritime Safety Committee which begins in May 2024.

OceanGate Expeditions, the company that owned and operated the Titan, is based in the US but the submersible was registered in the Bahamas. OceanGate is based in Everett, Washington, but closed when the Titan was found. Meanwhile, the Titan’s mothership, the Polar Prince, was from Canada, and those killed were from the UK, Pakistan, France, and the US.

The Coast Guard has convened a Marine Board of Investigation into the implosion. Photo / Paul Daly, The Canadian Press via AP

Killed in the implosion were Ocean Gate CEO and pilot Stockton Rush; two members of a prominent Pakistani family, Shahzada Dawood and his son Suleman Dawood; British adventurer Hamish Harding; and Titanic expert Paul-Henri Nargeolet.

The operator charged passengers $250,000 each to participate in the voyage. The implosion of the Titan has raised questions about the safety of private undersea exploration operations. The Coast Guard also wants to use the investigation to improve safety of submersibles.

- Patrick Whittle, AP